Publications

Lagenaur LA, Sanders-Beer BE, Brichacek B, Pal R, Liu X, Liu Y, Yu R, Venzon D, Lee PP, Hamer DH. Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus. Mucosal Immunol. 2011 Jul 6. [Epub ahead of print]

Stapleton AE, Au-Yeung M, Hooton TM, Fredricks DN, Roberts PL, Czaja CA, Yarova-Yarovaya Y, Fiedler T, Cox M, Stamm WE. (2011) Randomized, placebo-controlled phase 2 trial of a Lactobacillus crispatus probiotic given intravaginally for prevention of recurrent urinary tract infection. Clin Infect Dis. 52:1212-7.

Hemmerling A, Harrison W, Schroeder A, Park J, Korn A, Shiboski S, Foster-Rosales A, Cohen CR. (2010) Phase 2a study assessing colonization efficiency, safety and acceptability of Lactobacillus crispatus CTV-05 (LACTIN-V) in women with bacterial vaginosis. Sex Transm Dis. 2010 July 16 [Epub ahead of print]

Vangelista, L, Secchi M, Liu X, Bachi A, Jia L, Xu Q, Lusso P. (2010). Engineering of Lactobacillus jensenii to secrete RANTES and a CCR5 antagonist analogue as live HIV-1 blockers. .Antimicrob Agents Chemother. 54, 2994-3001.

Human Microbiome Jumpstart Reference Strains Consortium, Nelson KE, Weinstock GM, Highlander SK, Worley KC, Creasy HH, Wortman JR, Rusch DB, Mitreva M, Sodergren E, Chinwalla AT, Feldgarden M, Gevers D, Haas BJ, Madupu R, Ward DV, Birren BW, Gibbs RA, Methe B, Petrosino JF, Strausberg RL, Sutton GG, White OR, Wilson RK, Durkin S, Giglio MG, Gujja S, Howarth C, Kodira CD, Kyrpides N, Mehta T, Muzny DM, Pearson M, Pepin K, Pati A, Qin X, Yandava C, Zeng Q, Zhang L, Berlin AM, Chen L, Hepburn TA, Johnson J, McCorrison J, Miller J, Minx P, Nusbaum C, Russ C, Sykes SM, Tomlinson CM, Young S, Warren WC, Badger J, Crabtree J, Markowitz VM, Orvis J, Cree A, Ferriera S, Fulton LL, Fulton RS, Gillis M, Hemphill LD, Joshi V, Kovar C, Torralba M, Wetterstrand KA, Abouellleil A, Wollam AM, Buhay CJ, Ding Y, Dugan S, FitzGerald MG, Holder M, Hostetler J, Clifton SW, Allen-Vercoe E, Earl AM, Farmer CN, Liolios K, Surette MG, Xu Q, Pohl C, Wilczek-Boney K, Zhu D. (2010). A catalog of reference genomes from the human microbiome. Science. 328, 994-999.

Secchi M, Xu Q, Lusso P, Vangelista L. (2009). The superior folding of a RANTES analogue expressed in lactobacilli as compared to mammalian cells reveals a promising system to screen new RANTES mutants. Protein Expression and Purification 68, 34-41

Wang M, Song L, Yao L, Jia Y, Cheng H, Liu Y, Zhang, Z, Xu Q. (2009). Isolation and identification of vaginal lactobacilli from healthy 223 women of childbearing age. Chin J Clin Obstet Gynecol 10, 200-203

Hemmerling A, Harrison W, Schroeder A, Park J, Korn A, Shiboski S, Cohen CR. (2009) Phase 1 dose-ranging safety trial of Lactobacillus crispatus CTV-05 for the prevention of bacterial vaginosis. Sex Transm Dis. 36, 564-569.

Yu RR, Cheng AT, Lagenaur LA, Huang W, Weiss DE, Treece J, Sanders-Beer BE, Hammer DH, Lee PP, Xu Q, Liu Y. (2009) A Chinese rhesus macaque (Macaca mulatta) model for vaginal Lactobacillus colonization and live microbicide development. J Med Primatol. 38,125-136.

Liu X, Lagenaur LA, Lee PP, Xu Q. (2008) Engineering Human Vaginal Lactobacillus for Surface Expression of Two-Domain CD4. Applied and Environmental Microbiology 74, 4626-4635

Czaja CA, Stapleton AE, Yarova-Yarovaya Y, Stamm WE. (2007) Phase I trial of a Lactobacillus crispatus vaginal suppository for prevention of recurrent urinary tract infection in women. Infect Dis Obstet Gynecol. Article ID 353878.

Liu X, Lagenaur LA, Simpson DA, Essenmacher KP, Frazier-Parker CL, Liu Y, Tsai D, Rao SS, Hamer DH, Parks TP, Lee PP, Xu Q. (2006) Engineered Vaginal Lactobacillus for Mucosal Delivery of the HIV Inhibitor, Cyanovirin-N. Antimicrobial Agents and Chemotherapy 50, 3250-3259

Parks TP, Xu Q, Lagenaur L, Lee PP. (2005) Prevention and treatment of genital tract diseases, Chapter 8. In Probiotics and Prebiotics: Scientific Aspects, G.W. Tannock, ed. (Wymondham, UK: Horizon Scientific Press), pp. 171-194

Chang TL, Chang CH, Simpson DA, Xu Q, Martin PK, Lagenaur LA, Schoolnik GK, Ho DD, Hillier SL, Holodniy M, Lewicki JA, Lee PP. (2003) Inhibition of HIV infectivity by a natural human isolate of Lactobacillus jensenii engineered to express functional two-domain CD4. Proceedings of the National Academy of Sciences USA 100, 11672-11677

Recent Presentations

2010	Xu Q, Yu, R, Xia Q, W Huang, Jia L, Liu X, Liu Yang. Production and preservation of highly viable Lactobacillus for mucosal delivery of therapeutics in situ. Program and abstracts, Microbicides 2010, May 22-25, 2010. Fichorova R, Yamamoto H, Tang V, Yuan H, Liu Y, Jia L, Liu X, and Xu Q. Functional chacteristics of MucoCept Lactobacillus strains upon their adherence to human cervical and vaginal epithelial cells. Program and abstracts, Microbicides 2010, May 22-25, 2010. Hemmerling A, Harrison W, Schroeder A, Park J, Korn A, Foster-Rosales A, Shiboski S, Cohen CR. Phase 2 clinical trial testing colonization efficiency, safety and acceptability of Lactobacillus crispatus CTV-05 (LACTIN-V) in women for the prevention of recurrent bacterial vaginosis. Program and abstracts, Microbicides 2010, May 22-25, 2010. Ngugi B, Hemmerling A, Bukusi E, Kikuvi G, Gikunju J, Shiboski S, Fredericks D, Cohen C. Effects of BV-associated bacteria and sexual intercourse on vaginal colonization with the probiotic Lactobacillus crispatus CTV-05. Program and abstracts, Microbicides 2010, May 22-25, 2010. Liu Y, Yu R, Xia Q, Jia L, Liu X, Lee PP, and Xu Q. Formulation Development for Preservation of Lactobacillus as a Live Microbicide. Trends in Microbicide Formulations Formulations Workshop. Washington DC, Jan., 2010
2009	Liu X, Yu R, Liu Y, Lagenaur LA, Cheng A, Jia L, Xia Q, Hamer DH, Li J, Sanders-Beer BE, Lee PP, Xu Q. Mucosal Delivery of an Anti-HIV Molecule Cyanovirin-N by Lactobacillus in a Macaque Model, 27th annual Symposium on Nonhuman primate Models for AIDS, Boston, Oct. 28-31, 2009 Liu X, Liu Y, Rosa Yu, Laurel A. Lagenaur, Andrew Cheng, Letong Jia, Qing Xia, Dean H. Hamer, Brigitte E. Sanders, Peter P. Lee, and Qiang Xu. Vaginal Lactobacillus Bioengineered for Mucosal Delivery of the Anti-HIV Molecules. Advancing Prevention Technologies for Sexual and Reproductive Health: A Strategy Symposium in Berkeley, March, 2009 Lagenaur LA, Liu X, Liu Y, Rosa Yu, Andrew Cheng, Letong Jia, Qing Xia, Brigitte E. Sanders, Dean H. Hamer, Luca Vangelista, Paolo Lusso, Peter P. Lee, Qiang Xu. Live Microbicides - Human Vaginal Lactobacilli for Mucosal Delivery of Anti-HIV Proteins. Keystone Symposia on HIV Immunobiology: From Infection to Immune Control, March 2009 Stapleton AE, Au-Yeung M, Yarova-Yarovaya Y, Fredricks DN, Czaja C, Scholes D, Hooton TM, Stamm WE. Double-blind, placebo controlled phase 2 trial of LACTIN-V vaginal Lactobacillus crispatus probiotic to prevent recurrent UTI. Program and abstracts, 47th Annual Meeting of the IDSA, October 2009
2008	Liu Y, Yu R, Lagenaur LA, Cheng A, Weiss DE, Treece J, Sanders-Beer BE, Hamer DH, Lee PP, Xu Q. A Chinese rhesus macaque model for vaginal Lactobacillus colonization and live anti-HIV microbicide development, WHO Sponsored Symposium on Scientific, Regulatory and Public Health Aspects of Microbicide Research and Development, November 3-6, 2008, Nanjing, China Xu Q, Liu X, Liu Y, Lagenaur LA, Yu, R, Cheng A, Wang J, Xia Q, Jia L, Huang W, Sanders-Beer BE, Hamer DH, Lee PL. Vaginal Lactobacillus Bioengineered for Mucosal Delivery of Anti-HIV Inhibitors. WHO Sponsored Symposium on Scientific, Regulatory and Public Health Aspects of Microbicide Research and Development, November 3-6, 2008, Nanjing, China Xu Q, Liu X, Lagenaur LA, Liu Y, Yu, R, Cheng A, Wells, K, Wang J, Parks TP,, Hamer DH, Lee PL. Development of a live mucosal microbicide for women. An International Conference on Modern Mucosal Vaccines, Adjuvants and Microbicides, October 22-24, Tiara Park Altantico Proto, Portugal Xu Q, Liu X, Lagenauer LA, Liu Y, Yu RR, Wang J, Cheng A, Jia L, Huang W, Sanders-Beer BE, Hamer DH, LeePP. Vaginal Lactobacillus Bioengineered for Mucosal Delivery of Anti-HIV Microbicide, Cyanovirin-N. The 9th Symposium on Lactic Acid Bacteria, August 31 - September 4, 2008, Egmond aan Zee, The Netherlands Secchi M, Xu Q, Lusso, P. and Vangelista L. Secretion of the active form of the anti-HIV chemokine RANTES and a CCR5-antagonist analogue by Lactobacillus jensenii. Folding, assembly and secretion of complex protein entities, Recombinant Protein Production 2008. An international meeting held in Alghero, Sardinia, Italy, September, 2008 Vangelista L, Secchi M, Liu X, Xu Q, Lusso, P. Expression OF RANTES Derivatives in Lactobacilli: a novel strategy for the development of vaginal microbicides. Microbicides 2008 Conference, February 24-27, 2008, New Delhi, India Yu R, Lagenaur LA, Cheng A, Weiss DE, Treece J, Sanders-Beer BE, Hamer DH, Xu Q, Liu Y. A Chinese rhesus macaque (Macaca Mulatta) model for vaginal Lactobacillus colonization and live microbicide development, Microbicides 2008 Conference, February 24-27, 2008, New Delhi, India Xu Q, Liu X, Lagenaur LA, Liu Y, Yu R, Cheng A, Wells K, Wang J, Parks TP, Hamer DH, Lee PP. Development of a live topical microbicide for women. Microbicides 2008 Conference, February 24-27, 2008, New Delhi, India Stapleton AE, Au-Yeung M, Yarova-Yarovaya Y, Fiedler L, Fredricks DN, Czaja CA, Scholes D, Hooton TM, Stamm WE. Use of quantitative 16S rRNA gene PCR to assess vaginal colonization with a Lactobacillus crispatus probiotic. 48th Annual ICAAC and IDSA 46th Annual Meeting, Washington, DC, October 25-28, 2008, a. L-605, p. 583. Hemmerling A, Harrison W, Park J, Schroeder A, Korn A, Shiboski S, Cohen CR. A Phase I Dose-ranging Safety Trial Testing a Vaginal Probiotic (LACTIN-V) for the Prevention of Bacterial Vaginosis. 35th Annual Scientific Meeting of the Infectious Diseases Society for Obstetrics and Gynecology, Seattle, WA, August 14-16, 2008
2006	Xu Q, Lagenaur L, Liu X, Liu Y, Yu R, Wells K, Tsai D, Sweeney Y, Rao S, Hamer D, Patton D, Parks T, Lee P. Development of a live topical microbicide for women, International Meeting of the Institute of Human Virology, November 17-21, 2006, Baltimore, MD Lagenaur LA, Liu X, Wells K, Liu Y, Tsai D, Yu R, Zhu Y, Sweeney Y, Rao S, Hamer D, Parks TP, Patton D, Lee PP, Xu Q. Vaginal lactobacilli for mucosal delivery of the anti-HIV microbicide, Cyanovirin-N. Microbicides 2006 Conference, April 23-26, 2006, Cape Town, South Africa
2005	Xu Q, Lagenaur LA, Liu X, Simpson DA, Essenmacher K, Parker C, Chang CH, Tsai, D, Rao S, Hamer H, Parks TP, Lee PP. Vaginal lactobacilli for mucosal delivery of the anti-HIV microbicide, Cyanovirin-N (CV-N). International Meeting of the Institute of Human Virology, August 29 - September 2, 2005, Baltimore, MD, Lagenaur LA, Liu X, Simpson DA, Essenmacher K, Parker C, Chang CH, Tsai, D, Rao S, Hamer H, Parks TP, Lee PP, Xu Q. Development of vaginal lactobacilli for Mucosal Delivery of a topical microbicide, Cyanovirin-N (CV-N). International Meeting of the Institute of Human Virology, August 29-September 2, 2005, Baltimore, MD Lee PP, Liu X, Chang C, Lagenaur LA, Liu X, Parks TP, Xu Q. Native vaginal lactobacilli engineered as a delivery vehicle for anti-HIV protein microbicides. Joint Meeting of the 3 Division of the International Union of Microbiological Societies, July 23-28, 2005, San Francisco, CA Liu X, Lagenaur LA, Frazier-Parker CL Simpson DA, Chang CH., Tsai D, Essenmacher K, Lewicki JA, Parks TP, Lee PP, Xu Q. The MucoCept approach: a human vaginal isolate of Lactobacillus engineered to produce a potent anti-HIV microbicide. 8th Symposium on Lactic Acid Bacteria, August 28 - September 1, 2005, Egmond aan Zee, the Netherlands Lagenaur LA, Liu X, Simpson DA, Chang CH, Tsai D, Essenmacher K, Frazier-Parker CL, Lewicki JA, Lee PP, Parks TP, Xu Q. MucoCept: a Live Topical Microbicide for Women. Presented at Alliance for Microbicide Development Annual Meeting, January 13-14, 2005, Washington, DC.
2004	Lewicki JA, Simpson DA, Chang CH, Lagenaur LA, Essenmacher K, Parks TP, Lee PP, Martin PK, Liu X, Xu Q. Native vaginal lactobacilli engineered as a delievery vehicle for anti-HIV protein microbicides. Microbicides 2004 Conference, March 28-31, 2004, London, UK Chang CH, Cheng J, Frankel K, Essenmacher K, Simpson DA, Lagenaur LA, Parks TP, Martin PK, Liu X, Xu Q, Lewicki, JA. Optimizing expression of protein microbicides in lactobacilli: benefits of genomic sequencing, Microbicides 2004 Conference, March 28-31, 2004, London, UK
2003	Chang CH, Liu, X, Lagenaur LA, Lee PP, Xu Q, Lewicki JA. Surface expression of HIV-binding ligands in native vaginal flora as a potential approach to inhibit HIV-1 infectivity. Microbicide 2003 Conference, Washington DC
2002	Lagenaur, L., Chang, T., Simpson, D., Chang, C-H., Holodniy, M., Hillier S., Ho, D., Lee, P., Martin, P., Xu, Q., Lewicki, J. In vitro HIV neutralization wtih HIV-binding proteins secreted by an engineered human isolate Lactobacillus. The International Microbicides Conference, May, 2002, Antwerp